DBF4 anticorps (AA 295-665)

N° du produit ABIN7601162

Cet anticorps anti-DBF4 est un anticorps Lapin Polyclonal détectant DBF4 dans WB, ELISA, IF, ICC et FACS. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour DBF4 anticorps (AA 295-665) (ABIN7601162)

Antigène: DBF4 (DBF4 Homolog (DBF4))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DBF4 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-DBF4 anticorps

Épitope: AA 295-665

Fonction: Anti-DBF4 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-DBF4 Antibody Picoband® (ABIN7601162). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human DBF4 recombinant protein (Position:Y295-S665).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Brott, B. K., Sokol, S. Y. A vertebrate homolog of the cell cycle regulator Dbf4 is an inhibitor of Wnt signaling required for heart development. Dev. Cell 8: 703-715, 2005. Brott, B. K., Sokol, S. Y. A vertebrate homolog of the cell cycle regulator Dbf4 is an inhibitor of Wnt signaling required for heart development. Dev. Cell 8: 703-715, 2005. 2. Lepke, M., Putter, V., Staib, C., Kneissl, M., Berger, C., Hoehn, K., Nanda, I., Schmid, M., Grummt, F. Identification, characterization and chromosomal localization of the cognate human and murine DBF4 genes. Molec. Gen. Genet. 262: 220-229, 1999. Lepke, M., Putter, V., Staib, C., Kneissl, M., Berger, C., Hoehn, K., Nanda, I., Schmid, M., Grummt, F. Identification, characterization and chromosomal localization of the cognate human and murine DBF4 genes. Molec. Gen. Genet. 262: 220-229, 1999. 3. Zegerman, P., Diffley, J. F. X. Checkpoint-dependent inhibition of DNA replication initiation by Sld3 and Dbf4 phosphorylation. Nature 467: 474-478, 2010.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur DBF4

Antigène: DBF4 (DBF4 Homolog (DBF4))

Autre désignation: DBF4

Sujet:

Synonyms: Metabotropic glutamate receptor 5, mGluR5, GRM5, GPRC1E, MGLUR5

Tissue Specificity: Isoform 1 and isoform 2 are detected in bone marrow cells, spermatogonia and spermatocytes, but not in round spermatids, elongating spermatids and spermatozoa. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa, but not in spermatogonia and spermatocytes (at protein level). Isoform 1 is widely expressed and detected in fetal liver and bone marrow. Isoform 3 is detected in bone marrow cells enriched in hematopoietic stem cells.

Background: Using both gain- and loss-of-function experiments, it was found that Xenopus Dbf4 inhibited canonical Wnt signaling. Depletion of endogenous Dbf4 did not disturb gastrulation movements or early organizer genes, but resulted in embryos with morphologically defective heart and eyes and suppressed cardiac markers. The function of Dbf4 in heart development appeared to be independent of its role in the cell cycle. Dbf4 interacted physically and functionally with Frodo, a context-dependent regulator of Wnt signaling.

Poids moléculaire: 80 kDa

ID gène: 10926

Pathways: Mitotic G1-G1/S Phases, DNA Replication